Ribonuclease T1 from Aspergillus oryzae 核糖核酸酶T1
CAS 9026-12-4 MFCD00132191
化学结构图
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化学属性
| TSCA | No |
|---|
别名和识别编码
| MDL Number | MFCD00132191 |
|---|---|
| CAS Number | 9026-12-4 |
| Synonym | Guanyloribonuclease Guanyloribonuclease Ribonucleate 3′-guanylo-oligonucleotidohydrolase Guanyloribonuclease Ribonucleate 3'-guanylo-oligonucleotidohydrolase RNase F1 RNase G RNase Sa RNase T1 Ribonuclease T1 from Aspergillus oryzae Ribonucleate 3'-guanylo-oligonucleotidohydrolase |
| EC Number | 232-809-1 |
| Chemical Name | 来源于米曲霉(色谱纯化,冻干) Ribonuclease T1 from Aspergillus oryzae ammonium sulfate suspension, 300,000-600,000 units/mg protein |
| Chemical Name Translation | 核糖核酸酶T1 |
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分类
- {SNA} 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, Metabolomics, Nucleotide Metabolism, Protein & Nucl
- {SNA} 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Inde
产品应用
- Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .
安全信息
TYPE OF TEST : Mutation in microorganisms TEST SYSTEM : Bacteria - Salmonella typhimurium DOSE/DURATION : 100 mg/L REFERENCE : MGMVDU Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya. (Molekuliarnaia Genetika, Mikrobiologiia i Virusologiia, 117 GSP-7, Moskva B-246, USSR) No.1- 1983- Volume(issue)/page/year: 10,22,1991
其他信息
- Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.
