Deoxyribonuclease I From Bovine Pancreas 脱氧核糖核酸酶I来源于牛胰腺
CAS 9003-98-9 MFCD00130918
信息真实价格透明
资金保障
专业采购外包团队在线服务
信息真实价格透明
资金保障
专业采购外包团队在线服务
品牌质保精细包装
现货库存
一流品牌服务
分类
- {SNA} 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Enzymes for Diagnostic Kit Manufacturing, Molecular Diagnostics, Other Apoptosis Related Reagents, Protein & Nucleic Acid Isolation, 细胞信号转
产品应用
- 用于从蛋白质样品中除去 DNA。
- Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
相关文献及参考
- Merck: 14,2906
安全信息
GHS Symbol
- S24/25 Avoid contact with skin and eyes 避免皮肤和眼睛接触;
- P262 Do not get in eyes, on skin, or on clothing. 不要接触眼睛,皮肤或衣服。
TYPE OF TEST : Cytogenetic analysis TEST SYSTEM : Insect - Drosophila melanogaster Cells - not otherwise specified DOSE/DURATION : 1 gm/L REFERENCE : NULSAK Nucleus (Calcutta). (Dr. A.K. Sharma, Centre of Advanced Studies in Cell and Chromosome Research, Calcutta, 35 Baliygunge Circular Rd., Calcutta 700 019, India) V.1- 1958- Volume(issue)/page/year: 2,85,1959
TYPE OF TEST : Cytogenetic analysis TEST SYSTEM : Insect - grasshopper Cells - not otherwise specified DOSE/DURATION : 1 gm/L REFERENCE : NULSAK Nucleus (Calcutta). (Dr. A.K. Sharma, Centre of Advanced Studies in Cell and Chromosome Research, Calcutta, 35 Baliygunge Circular Rd., Calcutta 700 019, India) V.1- 1958- Volume(issue)/page/year: 2,85,1959
TYPE OF TEST : Sister chromatid exchange TEST SYSTEM : Rodent - hamster Ovary DOSE/DURATION : 2500 units REFERENCE : MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 307,315,1994
TYPE OF TEST : DNA damage TEST SYSTEM : Rodent - hamster Fibroblast DOSE/DURATION : 5000 ppm REFERENCE : SFCRAO Collection of Papers Presented at the Annual Symposium on Fundamental Cancer Research. (Baltimore, MD) V.1-29, 1946-76. For publisher information, see AFCPDR. Volume(issue)/page/year: 23,346,1970
其他信息
- 牛胰腺脱氧核糖核酸酶(又称DNA酶)是一种核酸内切酶,它作用于磷酸二脂键,特别针对毗邻嘧啶核苷的键,从而产生3' 末端带自由羟基和5' 末端带磷酸盐的多聚核苷酸。酶作用的最佳pH值为7.8。DNA酶能被二价金属活化,被鳌合物如EDTA和十二烷基硫酸钠抑制。浓度为5 mM的钙离子可作为稳定剂,以保护DNA酶不被水解酶分解。
- Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.