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• 直接询价

分类

• {SNA} Alkaline Phosphatase Substrates for Western Blotting, Alphabetical Listing, BCIP/NBT Substrate Systems, Colorimetric, Core Bioreagents, Detection, Detection Substrates, Enzyme Substrates, Life Science Reagents for Western Blotting, Research Essentials, Substrates, Tablets,

产品应用

• 碱性磷酸酶(alkaline phosphatase)的底物。
• 5-溴-4-氯-3-吲哚磷酸(BCIP) 和 氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛：包括 Northern Southern, Western blotting, 原位杂交（in situ hybridization）和免疫组织化学。
• BCIP 可以有两种盐的形式: 二钠盐型溶于水，对甲苯胺型溶于二甲基甲酰胺（DMF）。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液，构成碱性磷酸酶的底物溶液。 底物系统和碱性磷酸酶一起培养，产生一种不溶的带紫色的NBT双甲腊( diformazan )产物，容易被观察。

相关文献及参考

• McGadey, J., A tetrazolium method for non-specific alkaline phosphatase. Histochemie 23 , 180-184, (1970) 摘要
• Horwitz, J.P., et al. J. Med. Chem. 9 , 447, (1966)
• Leary, J.J., et al., Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc. Natl. Acad. Sci. U. S. A. 80 , 4045-4049, (1983)
• Knecht, D.A., Dimond, R.L., Visualization of antigenic proteins on Western blots. Anal. Biochem. 136 , 180, (1984)
• Walters, C., et al., Detection of parvovirus B19 in macerated fetal tissue using in situ hybridization. J. Clin. Pathol. 50(9) , 749-754, (1997) 摘要
• Meltzer, J.C., et al., Enhanced immunohistochemical detection of autonomic nerve fibers, cytokines and inducible nitric oxide synthase by light and fluorescent microscopy in rat spleen. J. Histochem. Cytochem. 45(4) , 599-610, (1997) 摘要

安全信息

其他信息

• 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/08/23 xw020009 5-溴-4-氯-3-吲哚磷酸酯对甲苯胺盐 (BCIP)（-20℃） 250mg 650元 2011/04/16 B1239 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐[用于生化研究] 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research] 1G 3120元 2011/04/16 B1239 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐[用于生化研究] 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research] 100MG 592元
• For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
• MOL 文件:6578-06-9.mol
• F:3-8-10
• Sigma Aldrich:6578-06-9(sigmaaldrich)
• TCI Shanghai:5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐[用于生化研究] 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research],>;98.0%(T)(6578-06-9)
• 用途一:碱性磷酸酶(alkaline phosphatase)的组织化学底物。5-溴-4-氯-3-吲哚磷酸(BCIP) 和 氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛：包括 Northern Southern, Western blotting, 原位杂交（in situ hybridization）和免疫组织化学。BCIP 可以有两种盐的形式: 二钠盐型溶于水，对甲苯胺型溶于二甲基甲酰胺（DMF）。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液，构成碱性磷酸酶的底物溶液。 底物系统和碱性磷酸酶一起培养，产生一种不溶的带紫色的NBT双甲腊( diformazan )产物，容易被观察。