Ethidium Bromide 溴化乙啶

CAS 1239-45-8 MFCD00011724

化学结构图

1239-45-8
SMILES: CC[n+]1c(-c2ccccc2)c2cc(N)ccc2c2ccc(N)cc21.[Br-]

化学属性

Mol. FormulaC21H20BrN3
Mol. Weight394
Melting Point260-262
TSCANo
Density1.156
Solubility40 G/L (25 ºC)
Flash Point>100°C
Appearance powder

别名和识别编码

Chemical NameEthidium Bromide
CAS Number1239-45-8
Synonym 2,7-DIAMINO-10-ETHYL-9-PHENYLPHENANTHRIDINIUM BROMIDE 2,7-DIAMINO-10-ETHYL-9-PHENYLPHENANTHRIDIUM BROMIDE 3,8-DIAMINO-5-ETHYL-6-PHENYLPHENANTHRIDINIUM BROMIDE 3,8-DIAMINO-5-ETHYL-6-PHENYLPHENANTHRIDIUM BROMIDE 3,8-Diamino-5-et 3,8-二氨基-5-乙 3,8-二氨基-5-乙基-6-苯基菲啶溴化物 EtBr 胡米溴铵 Bromide, Ethidium Bromide, Homidium CA NUMBERING: 3,8-DIAMINO-5-ETHYL-6-PHENYL-PHENANTHRIDINIUM BROMIDE EB;3,8-二氨基-5-乙基-6-苯基菲啶溴化物;2,7-二氨基-9-苯基氮杂菲-10-乙基溴;溴乙菲啶 Ethidium Ethidium Bromide HOMIDIUM BROMIDE Homidium Bromide Homidium bromide Homidium bromide 3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide Novidium RD 1572 溴乙锭(EB) 溴化乙啶 溴化乙啶 溶液 溴化乙锭 溴化乙锭, 98%(DRY WT.) 胡米溴铵 菲啶 菲啶溴红溶液
MDL NumberMFCD00011724
PubChem Substance ID14710
Beilstein Registry Number3642536
EC Number214-984-6
Reaxys-RN3642536
Merck Number4731
Chemical Name Translation溴化乙啶
Wiswesser Line NotationT B666 HKJ EJ H2 IR& LZ &E &9/26
LabNetwork Molecule IDLN00202612
InChIInChI=1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
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分类

  • {SNA} Detection, Fluorescent Enzyme Substrates, Fluorescent Enzyme Substrates for Electrophoresis, Fluorescent Stains, Nucleic Acid Electrophoresis Stains, Nucleic Acid Stains, Nucleic Acid Stains For Flow Cytometry, Nucleic Acid Stains For Microscopy, Nucleic Acid Stains For Spectroscopy, Wavelength Index, 发光化合物及其检测, 生化试剂, 荧光探针，标记，粒子和染色剂
  • {SNA} Agarose Blends for Electrophoresis, Apoptosis Detection, Apoptosis and Cell Cycle, Core Bioreagents, E-F, Ethidium Bromide, Fluorescent Stains, Nucleic Acid Electrophoresis Stains, Reagents, Research Essentials,

产品应用

  • 溴化乙锭是一种高度灵敏的荧光染色剂，用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号，可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 对于在电泳后进行凝胶染色，应先用水将储存液样品稀释到 0.5μg/ml，再将凝胶孵育 15-30 分钟。通常无需脱色，但是如果必须降低背景色，则可在水中脱色 15 分钟。然后可在紫外灯箱（254nm 波长）中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液（终浓度 0.5μg/ml）中，并在电泳后立即显影。

相关文献及参考

  • 3. Karsten, U. and Wollenberger, A., Improvements in the ethidium bromide method for direct fluorometric estimation of DNA and RNA in cell and tissue homogenates. Anal. Biochem. 77 , 464-470, (1977) 摘要
  • 4. Scaria, P.V. and Shafer, R.H., Binding of ethidium bromide to a DNA triple helix. Evidence for intercalation. J. Biol. Chem. 266 , 5417-5423, (1991) 摘要
  • , Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY , (1989) 6-44 , 6.15
  • Waring, M.J., et al.: J. Mol. Biol., 13, 269 (1965),
  • 2. El-Hamalawi, A.-R. A. et al., The fluorometric determination of nucleic acids in pea seeds by use of ethidium bromide complexes. Anal. Biochem. 67 , 384-391, (1975) 摘要
  • Probst, G.S., et al.: Environ. Mutagen., 3, 11 (1981),
  • Severini, A. and Morgan, A.R., An assay for proteinases and their inhibitors based on DNA/ethidium bromide fluorescence. Anal. Biochem. 193 , 83-89, (1991) 摘要
  • Reviews: J.-B. Le Pecq, Methods of Biochemical Analysis, vol. 20, Wiley, N.Y. (1971), p41; M. Waring, Antibiotics, vol. 3, Springer-Verlag (1975), p141.
  • 5. Elliott, W.H. Biochem. J. 86 , 562, (1963) 摘要
  • Intercalation agent for DNA.

安全信息

WGK Germany3
GHS Symbol
Precautionary statements
  • P201 Obtain special instructions before use. 使用前获取专门指示。
  • P202 Do not handle until all safety precautions have been read and understood. 已阅读并理解所有的安全预防措施之前，切勿操作。
  • P260 Do not breathe dust/fume/gas/mist/vapours/spray. 不要吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P264 Wash hands thoroughly after handling. 处理后要彻底洗净双手。
  • P270 Do not eat, drink or smoke when using this product. 使用本产品时不要吃东西，喝水或吸烟。
  • P271 Use only outdoors or in a well-ventilated area.? 只能在室外或通风良好的地方使用。
  • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
  • P281 Use personal protective equipment as required. 使用所需的个人防护装备。
  • P284 Wear respiratory protection.? 佩戴呼吸保护装置。
  • P301+P312
  • P301+P312+P330
  • P302+P350
  • P302+P352+P332+P313+P362+P364
  • P304+P340
  • P304+P340+P310
  • P305+P351+P338
  • P305+P351+P338+P337+P313
  • P308+P313
  • P310 Immediately call a POISON CENTER or doctor/physician. 立即呼救解毒中心或医生/医师。
  • P311 Call a POISON CENTER or doctor/physician. 呼叫解毒中心或医生/医师。
  • P320 Specific treatment is urgent (see … on this label). 紧急具体治疗（见本标签上的）。
  • P321 Specific treatment (see … on this label). 具体治疗（见本标签上的）。
  • P330 Rinse mouth. 漱口
  • P337+P313
  • P362 Take off contaminated clothing and wash before reuse. 脱掉污染的衣服，清洗后方可重新使用
  • P403+P233
  • P405 Store locked up. 上锁保管。
  • P501 Dispose of contents/container to..… 处理内容物/容器.....
Hazard statements
  • H302 Harmful if swallowed 吞食有害
  • H330 Fatal if inhaled 吸入致命
  • H331 Toxic if inhaled 吸入中毒
  • H341 Suspected of causing genetic defects 怀疑导致遗传性缺陷
Hazard Codes T+ T T,T+
Signal word
Personal Protective Equipment Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
RTECSSF7950000
UN Number UN 2811 6.1/PG 1 2811 1544
Safety Statements
Risk Statements
  • R68 Possible risk of irreversible effects 可能有不可挽回的作用的危险
  • R23 Toxic by inhalation 吸入有毒
  • R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
  • R21/22 Harmful in contact with skin and if swallowed 皮肤接触和不慎吞咽有害
  • R26 Very toxic by inhalation 吸入极毒
  • R22 Harmful if swallowed 吞咽有害
Packing GroupI
Storage condition 室温保存 2-8°C 室温保存. 0-10°C
Hazard Class6.1 
TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 20 mg/kg
TOXIC EFFECTS :
   Tumorigenic - active as anti-cancer agent
REFERENCE :
   CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut
   Sts., Philadelphia, PA 19106)  V.1-    1941-  Volume(issue)/page/year:
   34,2699,1974

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 110 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   ATMPA2 Annals of Tropical Medicine and Parasitology.  (Grune & Stratton,
   Inc., POB 6280, Duluth, MN 55806)  V.1-    1907-  Volume(issue)/page/year:
   46,285,1952

TYPE OF TEST            : DNA adduct
TEST SYSTEM             : Bird - wild bird species Leukocyte
DOSE/DURATION           : 37500 nmol/L
REFERENCE :
   BBRCA9 Biochemical and Biophysical Research Communications

TYPE OF TEST            : DNA damage
TEST SYSTEM             : Non-mammalian species
DOSE/DURATION           : 1 mol
REFERENCE :
   BBACAQ Biochimica et Biophysica Acta.  (Elsevier Science Pub. B.V., POB 211,
   1000 AE Amsterdam, Netherlands)  V.1-    1947- Volume(issue)/page/year:
   561,77,1979

TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Non-mammalian species Cells - not otherwise specified
DOSE/DURATION           : 10 mmol/L
REFERENCE :
   AAMMAU Archives d'Anatomie Microscopique et de Morphologie Experimentale.
   (SPPIF, B.P.22, F-41353 Vineuil, France) V.36-76, 1947-1987.  For publisher
   information,  see BSTME2.  Volume(issue)/page/year: 62,365,1973

TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Microorganism - not otherwise specified
DOSE/DURATION           : 5 umol/L
REFERENCE :
   JDREAF Journal of Dental Research.  (International Assoc. for Dental
   Research, 734 15th St., NW, Suite 809, Washington, DC 20005)  V.1-    1919-
   Volume(issue)/page/year: 55,266,1976

TYPE OF TEST            : DNA damage
TEST SYSTEM             : Microorganism - not otherwise specified
DOSE/DURATION           : 300 mg/L
REFERENCE :
   JMOBAK Journal of Molecular Biology.  (Academic Press, Inc., 1 E. First St.,
   Duluth, MN 55802) V.1-    1959-  Volume(issue)/page/year: 77,479,1973

TYPE OF TEST            : DNA inhibition
TEST SYSTEM             : Protozoa - Euglena gracilis
DOSE/DURATION           : 1 mg/L
REFERENCE :
   JNCSAI Journal of Cell Science.  (Biochemical Soc. Book Depot, POB 32,
   Commerce Way, Colchester, Essex CO2 8HP, UK)  V.1-    1966-
   Volume(issue)/page/year: 13,567,1973

其他信息

  • MSDS 信息:Homidium bromide(1239-45-8).msds
  • 用途二:溴化乙锭是一种高度灵敏的荧光染色剂，用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号，可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。
  • Alfa Aesar:溴化乙锭,98%(drywt.) Ethidium bromide, 98% (dry wt.)(1239-45-8)
  • 图谱信息:溴化乙啶(1239-45-8)红外图谱(IR1) 溴化乙啶(1239-45-8)红外图谱(IR2) 溴化乙啶(1239-45-8)质谱(MS)
  • MOL 文件:1239-45-8.mol
  • 荧光探针技术中的荧光探剂:溴化乙啶(简称EB)是目前在紫外线下观察DNA而常用的一种荧光染料，属于插入染料，原因是它有多环结构，这种结构使它能够插入核苷酸碱基之间。由于核酸和碱基的荧光量子产率很低，因此利用天然荧光检测核酸比蛋白质困难更大，所以荧光探针技术在核酸研究中显得尤为重要。比较常用的是溴化乙啶(et-hidium bromide或称菲啶溴红)，它与核酸 (DNA、RNA、双链多聚核苷酸)有特异结合的能力。它对脱氧核糖核酸的最低检测量可达10ng/ml，而且它与核酸的双链区结合是专一的，利用它与各种构象核酸结合比例的不同所产生的荧光强度的变化，可以把各种构型核酸加以区分。例如它能鉴别天然核酸和变性核酸，区分线性DNA、环状DNA和超级线团DNA等。此外各种吖啶，包括吖啶橙、吖啶黄以及前黄素等都是核酸常用的探剂。 荧光分析法可分为直接测定法和间接测定法二种。直接测定法是利用物质自身发射的荧光，即所谓“自荧光”或“内源荧光”来进行测定。但在自然界中只有少部分物质在光激发后可以发射荧光，而有相当一部分物质发荧光很弱或不发射荧光，如蛋白和核酸就是发荧光很弱的物质。为了充分利用荧光分析法灵敏度高这一特点就需使其转化成荧光物质再进行测定。此时可用某些试剂(如荧光染料) 使其与荧光较弱或不显荧光的物质共价或非共价结合，以形成发荧光的结合物再进行测定，这就是所谓的“荧光探针”技术或“荧光标记”技术。使试剂(荧光剂) 吸附在大分子上或共价结合到大分子上。所用的染料溴化乙啶被叫做“荧光探剂”。探剂荧光特性的变化，实际上是由于大分子构象的变化所引起的探剂环境变化的信号，所以利用这种信号还可以得到大分子构象变化的信息。
  • Sigma Aldrich:1239-45-8(sigmaaldrich)
  • 溴化乙啶价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/08/23 K4606501 溴化乙啶　生化荧光检测 1g 331元 2011/04/16 E0370 溴化乙啶 Ethidium Bromide 5G 1480元 2011/04/16 E0370 溴化乙啶 Ethidium Bromide 1G 499元
  • 外观性质:为芳香族荧光化合物。熔点:260 - 262℃
  • F:8-9
  • 海关编码:29339990
  • 为芳香族荧光化合物。 对于在电泳后进行凝胶染色，应先用水将储存液样品稀释?到 0.5μg/ml，再将凝胶孵育 15-30 分钟。通常无需脱色，但是如果必须降低背景色，则可在?水中脱色 15 分钟。然后可在紫外灯箱（254nm 波长）中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液（终浓度 0.5μg/ml）中，并在电泳后立即显影。
  • 为芳香族荧光化合物。
  • 用途一:EB不能穿过活细胞膜,可通过死细胞破损膜对细胞核DNA染色,含一个可嵌入堆积碱基之间的一平面基团,该基团固定位置及与碱基密切接近,致染料与DNA结合现荧光,因EB-DNA复合物荧光产率远大于未结合染料荧光,当电泳凝胶中含游离EB,可测少量DNA,是高灵敏荧光试剂,也是重要荧光染料,常测DNARNA.与DNA结合抑制DNA复制转录
  • TCI Shanghai:溴化乙啶 Ethidium Bromide,>;90.0%(T)(1239-45-8)
  • Acros Organics:溴化乙啶 Ethidium bromide, pure, 95%(1239-45-8)

系列性分类

  1. Research Essentials 基础科研
  2. Core Bioreagents - Research Essentials
  3. Life Science Reagents for DNA/RNA Electrophoresis - Core Bioreagents
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Fluorescent Probes, Labels, Particles and Stains - Biochemicals and Reagents
  4. Detection - Fluorescent Probes, Labels, Particles and Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Fluorescent Probes, Labels, Particles and Stains - Biochemicals and Reagents
  4. Fluorescent Stains - Fluorescent Probes, Labels, Particles and Stains
  5. Nucleic Acid Electrophoresis Stains - Fluorescent Stains
  1. Analytical & Chromatography 分析化学与色谱
  2. Analytical Reagents
  3. Microscopy Reagents - Analytical Reagents
  4. Stains and Dyes - Microscopy Reagents
  1. Life Science 生命科学
  2. Hematology and Histology 血液学和组织学
  3. Stains and Dyes - Hematology and Histology
  4. Stains & Dyes, A to Z - Stains and Dyes
  5. E-F - Stains & Dyes, A to Z
  1. Analytical & Chromatography 分析化学与色谱
  2. Analytical Standards
  3. Alphabetic - Analytical Standards
  4. P - Alphabetic
  5. PU - PZ - P
  1. Research Essentials 基础科研
  2. Core Bioreagents - Research Essentials
  3. Life Science Reagents for RNAi - Core Bioreagents
  4. General Reagents - Life Science Reagents for RNAi
  1. Life Science 生命科学
  2. Cell Biology 细胞生物学
  3. Cell Signaling and Neuroscience - Cell Biology
  4. Apoptosis and Cell Cycle - Cell Signaling and Neuroscience
  5. Apoptosis Detection - Apoptosis and Cell Cycle
  6. Reagents - Apoptosis Detection
  1. Research Essentials 基础科研
  2. Core Bioreagents - Research Essentials
  3. Agarose Blends for Electrophoresis - Core Bioreagents
  4. Ethidium Bromide - Agarose Blends for Electrophoresis
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Fluorescent Probes, Labels, Particles and Stains - Biochemicals and Reagents
  4. Fluorescent Stains - Fluorescent Probes, Labels, Particles and Stains
  5. Nucleic Acid Stains - Fluorescent Stains
  6. Nucleic Acid Stains For Spectroscopy - Nucleic Acid Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Chelators
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  4. Fluorescent Enzyme Substrates
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  4. Fluorescent Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Fluorescent Probes, Labels, Particles and Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Solvents, Buffers and Reagents for Luminescence
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Nucleic Acid Electrophoresis
  4. Agarose
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Nucleic Acid Electrophoresis
  4. Loading Buffers and Tracking Dyes
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Biochemicals and Reagents
  4. Stains and Dyes
  5. Natural Dyes
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Cell Signaling and Neuroscience
  4. Apoptosis and Cell Cycle
  5. Apoptosis Detection
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  4. Functional Group Reactive Labels
  5. Functional Group Reactive Labels for Spectroscopy
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Hematology and Histology
  4. Stains and Dyes
  5. Stains & Dyes, A to Z
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Hemotology and Histology
  4. Stains and Dyes
  5. Stains & Dyes, A to Z
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Fluorescent Probes, Labels, Particles and Stains
  5. Fluorescent Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Molecular Biology
  4. Nucleic Acid Electrophoresis
  5. Agarose Gel
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Molecular Biology
  4. Nucleic Acid Electrophoresis
  5. Loading Buffers and Tracking Dyes
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Cell Signaling and Neuroscience
  4. Peptides and Proteins
  5. Apoptosis and Cell Cycle Proteins
  6. Apoptosis Detection
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  4. Fluorescent Stains
  5. Nucleic Acid Stains
  6. Alphabetical
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  4. Fluorescent Stains
  5. Nucleic Acid Stains
  6. Nucleic Acid Stains For Flow Cytometry
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  4. Fluorescent Stains
  5. Nucleic Acid Stains
  6. Nucleic Acid Stains For Microscopy
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Fluorescent Probes, Labels, Particles and Stains
  5. Fluorescent Enzyme Substrates
  6. Alphabetical
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Proteins and Derivatives
  4. Proteins for Cell Biology
  5. Apoptosis and Cell Cycle Proteins
  6. Apoptosis Detection
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Fluorescent Probes, Labels, Particles and Stains
  5. Fluorescent Stains
  6. Nucleic Acid Stains
  7. Alphabetical
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Fluorescent Probes, Labels, Particles and Stains
  5. Fluorescent Stains
  6. Nucleic Acid Stains
  7. Nucleic Acid Stains For Flow Cytometry
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Fluorescent Probes, Labels, Particles and Stains
  5. Fluorescent Stains
  6. Nucleic Acid Stains
  7. Nucleic Acid Stains For Microscopy