• 原料直供
• 试剂商城
• 直接询价

分类

• {SNA} 2002/61/EC, Additional Standards, Alpha Sort, Analytical Standards, Chromatography, Environmental Standards, European Community: ISO, DIN and DL 152, Method and Regulation Specific, Organic Pollutant Standards, Q-Z, T-Z, Volatile & Semivolatile Standards, Volatiles/ Semivolatiles, 分析/色谱
• {SNA} Alphabetical Listing, Colorimetric, Detection, Detection Substrates, ELISA Substrates, Enzyme Substrates, Peroxidase, Peroxidase Substrates, Substrates by Enzyme, TMB Substrate Systems (3,3′,5,5′-Tetramethylbenzidine Substrate Systems),

产品应用

• 过氧化物酶和乙型肝炎抗原的染色剂。是一种新型安全的色原试剂。与传统的色原试剂联苯胺、邻联甲苯胺及邻苯二胺等相比较，TMB具有检测灵敏度高、稳定性好等优点，而且使用安全。经动物试验和Ames细菌自动变异性试验结果表明，TMB为非致癌物和非致突变物。目前TMB已在逐步取代强致癌物联苯胺和其他致癌性的联苯胺衍生物，应用于临床化验、法医检验、刑事侦破及环境监测等领域。尤其是在临床生化检验方面，TMB作为过氧化酶的新底物，在酶免疫分析法（EIA）和酶联免疫吸附检验法（ELISA）中获得了广泛的应用。
• (一)，TMB的主要应用项目如下：
• 1.指纹检测,是目前公安上显现潜血手印的最有效的方法
• 2.唾液中酒精的快速检测
• 3.尿联试纸的配制
• 4.肝炎病毒检测
• 5.妊娠检测试验
• 6.血液和尿液中葡萄糖、血红蛋白、白蛋白的快速测定
• 7.粪便隐血试验
• 8.血液中粒细胞值的测定
• 9.类固醇、性激素的检测
• 10.酶活性的测定
• 11.抗原、抗体及遗传物质的分析、检测
• 12.生物样品的染色
• 13.水质检测
• 14、测定商品中偶氮染料释放的芳香胺的标准

相关文献及参考

• 1. Holland, V.R., et al. Tetrahedron 30 , 3299, (1974)
• 2. D.D. Garner et al. J. Forensic Sci. 21 , 819, (1976)
• 3. J.C. Standefer, D. Vanderjagt Clin. Chem. 23 , 749, (1977)
• 4. R.C. Lijana, M.C. Williams J. Lab. Clin. Med. 94 , 266, (1979)
• 5. Liem, H.H. et al. Anal. Biochem. 98 , 388, (1979)
• 6. Bos, E.S., et al. J. Immunoassay 2 , 187, (1981)
• 7. P.C. Andrews, N.I. Krinsky Anal. Biochem. 127 , 346, (1982)
• 8. H. Gallati, I. Pracht J. Clin. Chem. Clin. Biochem. 23 , 453, (1985)
• Giorgio, C., et al., Lithocholic Acid Is An Eph-ephrin Ligand Interfering With Eph-kinase Activation. PLoS ONE 6 , e18128, (2011) 摘要
• Aldrich MSDS 1 , 1679:B / Corp MSDS 1 (2), 3292:A / FT-IR 2 (2), 2090:C / FT-IR 1 (1), 1236:D / FT-NMR 1 (2), 529:B / FT-NMR 1 (1), 1236:D / IR-Spectra (3), 740:D / RegBook 1 (1), 1423:J / Sigma FT-IR 1 (2), 218:D / Stains and Dyes Ref , 693 / Structure Index 1 , 222:D:3

安全信息

GHS Symbol

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Oral
SPECIES OBSERVED        : Bird - quail
DOSE/DURATION           : >316 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   EESADV Ecotoxicology and Environmental Safety.  (Academic Press, Inc., 1 E.
   First St., Duluth, MN 55802)  V.1-    1977-  Volume(issue)/page/year:
   6,149,1982

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 135 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   PMRSDJ Progress in Mutation Research.  (Elsevier Science Pub. Co., Inc., 52
   Vanderbilt Ave., New York, NY 10017)  V.1-    1981- Volume(issue)/page/year:
   1,682,1981

TYPE OF TEST            : Micronucleus test
ROUTE OF EXPOSURE       : Intraperitoneal
TEST SYSTEM             : Rodent - mouse
DOSE/DURATION           : 112500 ug/kg
REFERENCE :
   PMRSDJ Progress in Mutation Research.  (Elsevier Science Pub. Co., Inc., 52
   Vanderbilt Ave., New York, NY 10017)  V.1-    1981- Volume(issue)/page/year:
   1,698,1981

TYPE OF TEST            : DNA adduct
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 20 umol/L
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 89,95,1981

TYPE OF TEST            : Mutation in mammalian somatic cells
TEST SYSTEM             : Rodent - mouse Lymphocyte
DOSE/DURATION           : 30700 ug/L
REFERENCE :
   EMMUEG Environmental and Molecular Mutagenesis.  (Alan R. Liss, Inc., 41 E.
   11th St., New York, NY 10003)  V.10-    1987-  Volume(issue)/page/year:
   12(Suppl 13),37,1988

其他信息

• TMB作为过氧化氢酶底物用在ELISA 反应中，该底物能产生可溶性淡蓝色的末端产物，能在370 或 620-650 nm用分光光度计读取。TMB反应可以用2 M H2SO4 (产生黄色)终止, 并能在450 nm读取。因此TMB是测定血液、检测血红素（hemoglobin）和过氧化物酶的灵敏和特定试剂。