Collagenase from Clostridium histolyticum 胶原蛋白酶
CAS 9001-12-1 MFCD00130830
化学结构图
SMILES: CCCCCCCCC1CCC2(C)C3CCC4(C)Cc5nc6c(nc5CC4(C)C3(C)CCC12C)CC1(C)CCC2C3(C)CCC(CCCCCCCC)C3(C)CCC2(C)C1(C)C6
别名和识别编码
| Synonym | Aspergillopeptidase C; Azocollase; Clostridiopeptidase A; Clostridiopeptidase I; Clostridiopeptidase II; Clostridium histolyticum collagenase; Collagen peptidase; Collagen protease; Collagenase A; Matrix metalloprotease-1; Metallocollagenase; Santyl; Soycollagestin 梭菌肽酶 A Matrix Metalloproteinase-1 人 胶原蛋白酶 来源于溶组织梭菌 Collagenase-1 Interstitial Collagenase MMP-1 |
|---|---|
| MDL Number | MFCD00130830 |
| CAS Number | 9001-12-1 |
| EC Number | 232-582-9 |
| Chemical Name | Collagenase from Clostridium histolyticum |
| Chemical Name Translation | 胶原蛋白酶 |
| PubChem Substance ID | 44384985 |
| InChI | InChI=1S/C60H100N2/c1-13-15-17-19-21-23-25-43-27-33-55(7)49-29-31-51(3)39-45-47(41-59(51,11)57(49,9)37-35-53(43,55)5)61-46-40-52(4)32-30-50-56(8)34-28-44(26-24-22-20-18-16-14-2)54(56,6)36-38-58(50,10)60(52,12)42-48(46)62-45/h43-44,49-50H,13-42H2,1-12H3/t43-,44-,49?,50?,51-,52-,53+,54+,55-,56-,57-,58-,59-,60-/m0/s1 |
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分类
- {SNA} 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Dissociation, Cell Dissociation and Cell Lysis, Cell Signaling Enzymes, Cell Signaling and Neuroscience, Collagenases, Core Bioreagents, Crude Collagenase, Use-Tested, Cytoskeleton and Extracellular Matrix, Enzyme Class Index, Enzymes, Enzymes for Diagnostic Kit Manufacturing, Enzymes, Inhibitors, and Substrates, Extracellular Matrix, Extracellular Matrix Peptides and Proteins, Histology, MMP Enzymes, Matrix Metalloproteinases Products, Proteases, Proteases & Protein
产品应用
- This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.
相关文献及参考
- Templeton, N.S., et al., Cloning and characterization of human tumor cell interstitial collagenase. Cancer Res. 50 , 5431-5437, (1990) 摘要
- Cawston, T.E., Barrett, N.D., et al., Interstitial collagenase. Handbook of Proteolytic Enzymes San Diego , (2004), 472-480
安全信息
GHS Symbol
- P342+P311
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
- P305+P351+P338
- H315 Causes skin irritation 会刺激皮肤
- H319 Causes serious eye irritation 严重刺激眼睛
- H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled 吸入可能导致过敏或哮喘症状或呼吸困难
- H335 May cause respiratory irritation 可能导致呼吸道刺激
- S24 Avoid contact with skin 避免皮肤接触；
- S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后，立即用大量水冲洗并征求医生意见；
- S36/37 Wear suitable protective clothing and gloves 穿戴适当的防护服和手套；
- S22 Do not breathe dust 不要吸入粉尘；
- R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
- R42 May cause sensitisation by inhalation 吸入会产生过敏反应
其他信息
- Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.
