3-Amino-9-ethylcarbazole 3-氨基-9-乙基咔唑
CAS 132-32-1 MFCD00004964
化学结构图
SMILES: CCn1c2ccccc2c2cc(N)ccc21
化学属性
| Mol. Formula | C14 |
|---|---|
| Mol. Weight | 210 |
| Density | 1.17 |
| Melting Point | 98-100 |
| TSCA | Yes |
| Appearance | tablet |
别名和识别编码
| Chemical Name | 3-Amino-9-ethylcarbazole |
|---|---|
| MDL Number | MFCD00004964 |
| CAS Number | 132-32-1 |
| EC Number | 205-057-7 |
| Beilstein Registry Number | 22(4)4936 |
| Synonym | 3-amino-n-ethylcarbazol 9-ethyl-9h-carbazol-3-amin 9-ETHYLCARBAZOL-3-AMINE 9-Ethyl-9H-carbazol-3-amine AEC BUFFER 3-Amino-9-ethylcarbazole (AEC) 3-氨基-N-乙基咔唑 3AC |
| PubChem Substance ID | 87560684 |
| Reaxys-RN | 174969 |
| Alfabeta Name | ETHYLCARBAZOLAMINE H993----- |
| Chemical Name Translation | 3-氨基-9-乙基咔唑 |
| InChI | InChI=1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3 |
| LabNetwork Molecule ID | LN00181685 |
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分类
- {SNA} Alphabetical, Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Core Bioreagents, Detection, Detection Substrates, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Fluorescent Enzyme Substrates, Fluorescent Probes, Labels, Particles and Stains, Life Science Reagents for Western Blotting, Peroxidase Subs
- {SNA} Alphabetical, Alphabetical Listing, Colorimetric, Detection, Detection Substrates, Enzyme Substrates, Fluorescent Enzyme Substrates, Peroxidase Substrates, Substrates by Enzyme, 生化试剂, 荧光探针，标记，粒子和染色剂, 酶、抑制剂和底物
产品应用
- 过氧化物酶底物.
- Usage
- ? Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps.
- ? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;).
- ? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well.
- ? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT.
- ? Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain.
- ? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; )
- ? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations
相关文献及参考
- Graham, R.C., et al. J. Histochem. Cytochem. 13 , 150, (1965)
- Kaplow, L.S. Am. J. Clin. Pathol. 63 , 451, (1974)
- Beil. 22 ,IV,4936
- Aldrich MSDS 1 , 80:A / Corp MSDS 1 (1), 169:D / FT-IR 2 (3), 3613:B / FT-IR 1 (2), 680:D / FT-NMR 1 (3), 163:A / RegBook 1 (2), 2425:I / Sax 6 , 214 / Sigma FT-IR 1 (2), 265:D / Structure Index 1 , 384:B:8
安全信息
GHS Symbol
- S45 In case of accident or if you feel unwell seek medical advice immediately (show the label where possible) 发生事故时或感觉不适时，立即求医（可能时出示标签）；
- S53 Avoid exposure - obtain special instructions before use 避免接触，使用前获得特别指示说明；
- S36/37/39 Wear suitable protective clothing, gloves and eye/face protection 穿戴适当的防护服、手套和眼睛/面保护；
- S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后，立即用大量水冲洗并征求医生意见；
- R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
- R25 Toxic if swallowed 吞咽有毒
- R40 Limited evidence of a carcinogenic effect 有限证据表明其致癌作用
- R45 May cause cancer 可能致癌
- P201 Obtain special instructions before use. 使用前获取专门指示。
- P202 Do not handle until all safety precautions have been read and understood. 已阅读并理解所有的安全预防措施之前，切勿操作。
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
- P264 Wash hands thoroughly after handling. 处理后要彻底洗净双手。
- P270 Do not eat, drink or smoke when using this product. 使用本产品时不要吃东西，喝水或吸烟。
- P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
- P301+P310
- P301+P310+P330
- P305+P351+P338
- P308+P313
- P405 Store locked up. 上锁保管。
- P501 Dispose of contents/container to..… 处理内容物/容器.....
- H301 Toxic if swallowed 吞食有毒
- H350 May cause cancer 可能致癌
其他信息
- 上游原料:乙醇 --> 盐酸 --> 甲醇 --> 乙酸 --> N,N-二甲基甲酰胺 --> 硝酸 --> 氢氧化铵 --> 铁粉 --> 草酸二乙酯 --> 硫化钠 --> 叔丁醇钾 --> 咔唑 --> 3-硝基-N-乙基咔唑
- MOL 文件:132-32-1.mol
- 储运特性:库房通风低温干燥
- 3-氨基-9-乙基咔唑价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2011/04/16 A2167 3-氨基-9-乙基咔唑[用于生化研究] 3-Amino-9-ethylcarbazole [for Biochemical Research] 1G 260元 2011/04/16 A2167 3-氨基-9-乙基咔唑[用于生化研究] 3-Amino-9-ethylcarbazole [for Biochemical Research] 5G 779元 2011/04/16 A1508 3-氨基-9-乙基咔唑 3-Amino-9-ethylcarbazole 5G 378元
- AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine
- Alfa Aesar:3-氨基-9-乙基咔唑,95% 3-Amino-9-ethylcarbazole, 95%(132-32-1)
- AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer .
- Acros Organics:3-氨基-9-乙基卡唑 3-Amino-9-ethylcarbazole, tech., 90%(132-32-1)
- 毒性分级:中毒
- 方法一:由3-硝基-N-乙基咔唑用硫化钠还原而得。
- 下游产品:颜料紫 23 --> C.I.直接蓝108
- 3-氨基-9-乙基咔唑价格(试剂级):更新日期 产品编号 产品名称 包装 价格 2014/06/02 A2167 3-氨基-9-乙基咔唑[用于生化研究] 3-Am
- 用途三:一种过氧化酶底物，适用于免疫印迹和免疫组织化学染色程序。该底物能产生一种不溶性红色的末端产物。肉眼或可见光下就可观察。
- 用途一:用作染料中间体
